Купить СНПЧ А7 Архангельск, оперативня доставка

crosscheckdeposited

Light / Dark Preference in Danio rerio: Effects of Light Exposure Duration and Day Period

DOI: http://dx.doi.org/10.18561/2179-5746/biotaamazonia.v4n3p106-111

http://periodicos.unifap.br/index.php/biota/index 

downloadpdf

Cláudio A. G. de M. Dias1,2*, Bruno R. dos Santos1, Bruno de M. Mansur1, Marcelo de S. Pinheiro1, Amanda A. Fecury3, Julio C. Sá-Oliveira2, Paulo H. Matayoshi-Calixto2, Euzébio de Oliveira4 & Amauri Gouveia Júnior1

 

Abstract: The animals have anatomical and physiological structures influenced by luminosity. In fish, the pineal gland cells contain photoreceptors and the suprachiasmatic nucleus seems to be involved in biological rhythm just like in other vertebrates. This work aims to describe the role of luminosity and day periods in Danio rerio's Light/Dark preference. For this purpose 160 naive adult undetermined sex Zebrafish were divided into 5 groups of 32 subjects and kept in isolated aquaria with light control. Afterwards, each fish was tested in the Light/Dark aquaria, with water column of 5 centimeters. There was an habituation period of 5 minutes (300 seconds) followed by removal of the sliding doors, allowing the subjects to explore the apparatus for 15 minutes (900 seconds). Tests were performed in different day periods, as follows: Morning (06:00 – 12:00 h); Afternoon (12:00 – 18:00 h); Night (18:00 – 24:00 h); Late Night (24:00 – 06:00 h). Our results show that the time of permanence is sensitive to day period. The number of crossings and latency were not influenced by the period of the day. Further studies regarding biological basis of Light/Dark Preference should be carried out in order to understand the role of circadian function in Danio rerio's behavior.

Key words: Danio rerio, zebrafish, behavior, light-dark preference, anxiety.

 

Resumo: Os animais têm estruturas anatômicas e fisiológicas influenciadas pela luminosidade. Nos peixes, as células da glândula pineal contém fotorreceptores e o núcleo supraquiasmático parece estar envolvido no ritmo biológico, assim como em outros vertebrados. Este trabalho tem como objetivo descrever o papel da luminosidade e dos períodos do dia na preferência claro/escuro em Danio rerio. Para isto 160 animais adultos, ingênuos, de sexo indeterminado foram divididos em 5 grupos de 32 indivíduos e mantidos em aquários isolado com controle de luminosidade. Depois, cada peixe foi testado no aquário claro/escuro, com coluna de água de 5 centímetros. Houve um período de habituação de 5 minutos (300 segundos), seguido por remoção das portas deslizantes, permitindo aos indivíduos que explorarem o aquario durante 15 minutos (900 segundos). Os testes foram realizados em períodos diferentes do dia: Manhã (6:00-00:00 h); Tarde (12:00-18:00 h); Noite (18:00 - 24:00 h); Madrugada (24:00 h - 06:00 h). Os resultados mostram que o tempo de permanência é sensível a períodos do dia. O número de cruzamentos e latência não foram influenciados pelo período do dia. Mais estudos sobre bases biológicas da preferência claro/escuro devem ser realizadas a fim de entender o papel da função circadiana no comportamento de Danio rerio.

Palavras-chave: Danio rerio, paulistinha, comportamento, preferência claro-escuro, ansiedade.

 

1 Laboratory of Neuroscience and Behavior, Nucleus for Theory and Behavioral Research, Federal University of Pará, Brazil
2 Nucleus for Study on Fisheries and Aquaculture, Federal University of Amapá, Brazil
3 Laboratory of Clinical Pathology and Tropical Diseases , Tropical Medicine Nucleus, Federal University of Pará, Brazil
4 Federal University of Pará, Brazil.
* Corresponding Author: Cláudio Alberto Gellis de Mattos Dias. Rua Emanoel Souza e Silva, 223, Apto 107, Jardim Equatorial, CEP 68903-003 Macapá, AP +55 91 3201 8485 / +55 91 8357 3726. E-mail: claudiodias@unifap.br

 

Literatura Citada

ARAÚJO, A. P. Q. C. Doenças metabólicas com manifestações psiquiátricas. Rev. Psiq. Clín. , v. 31 (6), p. 285-289, 2004.

BILOTTA, J.; SASZIK, S. The zebrafish as a model visual system. Int. J. Devl Neuroscience, v. 19, p. 621–629, 2001.

BOURIN, M.; HASCOEÜT, M. The mouse light/dark box test. European Journal of Pharmacology, v. 463, p. 55-65, 2003.

CARDOSO, F. R. G. et al. A simple model for circadian timing by mammals. Braz J Med Biol Res, v. 42, n. 1, p. 122 - 127, 2009.

CHAOULOFF, F.; DURAND, M.; MORMÀDE, P. Anxiety- and activity- related effects of diazepam and chlordiazepoxide in the rat light/dark and dark/light tests. Behavioural Brain Research, v. 85, p. 27-35, 1997.

COSTALL, B. et al. Exploration of Mice in a Black and White Test Box: Validation as a Model of Anxiety. Pharmacology Biochemistry & Behavior, v. 32, p. 777-785, 1989.

EVANS, D. H. The Physiology of Fishies. CRC Press, 1998.

FLEISCH, V. C.; NEUHAUSS, S. C. F. Visual Behavior in Zebrafish. Zebrafish, v. 3, n. 2, 2006.

FLINN, L. et al. Zebrafish as a new animal model for movement disorders. J. Neurochem., v. 106, p. 1991–1997, 2008.

FONKEN, L. K. et al. Influence of light at night on murine anxietyand depressive-like responses. Behavioural Brain Research, v. 205, p. 349–354, 2009.

GOUVEIA JR, A. et al. Preference of goldfish (Carassius aurautus) for dark places. Revista de Etologia, v. 7, n. 2, p. 63-66, 2005.

HOUART, C. Zebrafish as an Experimental Organism. Encyclopedia of Life Sciences, p. 1 - 5, 2001.

HURD, M. W. et al. Circadian rhythms of locomotor activity in zebrafish. Physiology & Behavior, v. 65, n. 3, p. 465–472, 1998.

KAZIMI, N.; CAHILL, G. M. Development of a circadian melatonin rhythm in embryonic zebrafish. Developmental Brain Research, v. 117, p. 47–52, 1999.

KRAUSE, J.; HARTMANN, N.; PRITCHARD, V. L. The influence of nutritional state on shoal choice in zebrafish, Danio rerio. Animal Behaviour, v. 57, p. 771–775, 1999.

KREBS, J. R.; DAVIES, N. B. Introdução à ecologia comportamental. Atheneu Editora, 1996.

LÓPEZ-OLMEDA, J. F.; MADRID, J. A.; SÁNCHEZ-VÁZQUEZ, F. J. Melatonin effects on food intake and activity rhythms in two fish species with different activity patterns: Diurnal (goldfish) and nocturnal (tench). Comparative Biochemistry and Physiology, Part A v. 144 p. 180–187, 2006.

LORENC-DUDA, A. et al. Turkey retina and pineal gland diVerentially respond to constant environment. J Comp Physiol, v. 194, p. 907–913, 2008.

LUCHIARI, A. C. et al. Light intensity preference of juvenile pikeperch Sander lucioperca (L.). Aquaculture Research, v. 37, p. 1572-1577, 2006.

MAXIMINO, C. et al. A Comparative Analysis of the Preference for Dark Environments in Five Teleosts. International Journal of Comparative Psychology, v. 20, p. 351-367, 2007.

MAXIMINO, C. et al. Scototaxis as anxiety-like behavior in fish. Nature Protocols, v. 5, p. 221-228, 2010.

MESQUITA, F. O. et al. A preliminary study into the effectiveness of stroboscopic light as an aversive stimulus for fish. Applied Animal Behaviour Science, v. 111, p. 402–407, 2008.

PLAUT, I. Effects of fin size on swimming performance, swimming behaviour and routine activity of zebrafish Danio rerio. The Journal of Experimental Biology, v. 203, p. 813–820, 2000.

PRITCHARD, V. L. et al. Shoal choice in zebrafish, Danio rerio: the influence of shoal size and activity. Animal Behaviour, v. 62, p. 1085–1088, 2001.

RUHL, N.; MC ROBERT, S. P. The effect of sex and shoal size on shoaling behaviour in Danio rerio. Journal of Fish Biology, v. 67, p. 1318–1326, 2005.

SERRA, E. L.; MEDALHA, C. C.; MATTIOLI, R. Natural preference of zebrafish (Danio rerio) for a dark environment. Brazilian Journal of Medical and Biological Research, v. 32, p. 1551- 1553, 1999.

SPENCE, R. et al. The distribution and habitat preferences of the zebrafish in Bangladesh. Journal of Fish Biology, v. 69, p. 1435–1448, 2006.

SPENCE, R. et al. The behaviour and ecology of the zebrafish, Danio rerio. Biological Reviews, v. 83 p. 13–34, 2008. WRIGHT, D. et al. Inter and intra-population variation in shoaling and boldness in the zebrafish (Danio rerio) . Naturwissenschaften, v. 90, p. 374–377, 2003.