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Characterization of Dentin Surface After Application of Desensitizing Agents: SEM Analysis

DOI: http://dx.doi.org/10.18256/2238-510X/j.oralinvestigations.v4n1p33-38

https://seer.imed.edu.br/index.php/JOI/index 

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Sandra K. Moura1, Tania C. Simões1,2, Carolina C. Lopes1, Claudia S. Silva1, André T. G. Sá2 & Linda Wang3

  

Abstract: Objective: To analyze the morphology of dentin after application of desensitizing agents. Materials and Methods: Ten third molars were sectioned to obtain 2mm thick dentin disks and divided into 4 groups (n = 5): G0 – cut with a diamond disk, G1 – Colgate® Sensitive Pro-relief™ toothpaste; G2 – Duraflur ® Fluoride varnish; G3 – biosilicate aqueous solution (Biosilicato®). After storage in dehydrated silica-gel (37°C/24h), the samples were placed on aluminum supports, coated with gold-palladium and observed under a scanning electron microscope. Post-treatment photomicrographs were obtained of the dentin. Results: In the G1 group the smear layer was observed; in the G2 and G4 groups, both the desensitizing agents and the smear layer and in the G3 group, a film of varnish. Conclusions: Different morphologies of dentin were observed after the proposed treatments for dentin hypersensitivity.

Key words: Dental Scaling, Dentin sensitivity, Desensitizing agents, Scanning electron microscopy.

 

Resumo: Objetivo: Observar a morfologia da dentina após aplicação de agentes dessensibilizantes. Método: Dez terceiros molares foram secionados para obter discos de dentina de 2mm de espessura, divididos em 4 grupos (n = 5): G1 – corte com disco diamantado, G2 – creme dental Colgate® Sensitive Pro-Alívio™; G3 – verniz fluoretado Duraflur®; G4 – solução aquosa de biosilicato (Biosilicato®). Após os tratamentos e armazenagem em silica-gel desidratada (37°C/24h), as amostras foram posicionadas em suportes de alumínio, cobertas com liga de ouro-paládio e observadas em microscópio eletrônico de varredura para obtenção de fotomicrografias da dentina. Resultados: Foram observados: smear layer no grupo G1; agentes dessensibilizantes e smear layer nos grupos G2 e G4 e no grupo G3 a película de verniz. Conclusão: Foram observados aspectos morfológicos distintos nas superficies da dentina tratada pelos agentes dessensibilizantes utilizados no tratamento da sensibilidade dentinária

Palavras-chave: Raspagem dental, Sensibilidade da dentina, Dessensibilizantes, Microscopia eletrônica de varredura.

 

1 Departamento de Dentística, Universidade Norte do Paraná, Londrina, PR, Brasil
2 Instituto Federal do Paraná, Londrina, PR, Brasil
3 Departamento de Dentística, Faculdade de Odontologia de Bauru, Universidade de São Paulo, Bauru, SP, Brasil

 

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