Купить СНПЧ А7 Архангельск, оперативня доставка

crosscheckdeposited

In Vitro Adventitious Shoot Regeneration from Sweet Orange Using Thin Epicotyl Sections

DOI: http://dx.doi.org/10.13082/1984-7033.v01n01a03

http://www.sbmp.org.br/cbab/siscbab/ 

downloadpdf

João C. Bespalhok Filho1, Adilson K. Kobayashi2, Luiz F. P. Pereira2, Zuleide Hissano2 & Luiz G. E. Vieira2

 

Abstract: A system for in vitro plant regeneration from thin transversal epicotyl sections of sweet orange (Citrus sinenis L. Osbeck cvs Pera, Valencia and Folha Murcha) was developed. Thin section explants (1-2 mm) from seedlings produced adventitious shoot buds on MS medium supplemented with BA (0.5-5 μM). Optimal shoot induction was achieved on 2-5 M BA. Explants with buds were transferred to elongation media in order to improve the recovery of normal shoots. Higher number of elongated shoots was obtained on medium with 0.5 μM BA. Elongated shoots were rooted on half strength MS medium without growth regulators and transferred to soil for acclimatization. The advantages of this system for transformation are discussed.

Key words: Citrus sinensis, adventitious buds, shoot multiplication

 

1 Departamento de Agronomia, Universidade Estadual de Londrina (UEL), C.P. 6001, CEP 86051-990, Londrina – Paraná, Brazil
2 Instituto Agronômico do Paraná (IAPAR), C. P. 481, CEP 86001-970, Londrina – Paraná, Brazil

 

Literatura Citada

Balch, E.P.M. and Alejo, N. O. 1997. In vitro plant regeneration of Mexican lime and mandarim by direct organogenesis. Hortscience. 32:931-934.

Bhagwat, B.; Vieira, L. G. E. and Erickson, L. R. 1996. Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberelic acid. Plant Cell Tissue and Organ Culture. 46:1-7. doi

Cervera, M.; Juárez, J.; Navarro, A.; Pina, J.; Navarro, L. and Peña, L. 1998. Genetic transformation of woody fruit plants bypassing the juvenile stage. Transgenic Research. 7:51-59. doi

Duran-Vila, N.; Ortega, V. and Navarro, L. 1989. Morphogenesis and tissue culture of three citrus species. Plant Cell Tissue and Organ Culture. 16:123-133. doi

Gill, M. I. S.; Singh, Z.; Dhillon, B. S. and Gosal, S. S. 1995. Somatic embryogenesis and plantlet regeneration in mandarin (Citrus reticulata Blanco). Scientia Horticulturae. 63:167-174. doi

Gmitter Jr, F. G.; Grosser, J. W. and Moore, G. A. 1992. Citrus. p.335-369. In: Hammerschlag FA and Litz RE (Eds). Biotechnology of perennial fruit crops. Biotechnology in Agriculture n.8. CAB International, Cambridge.

Gutiérrez-E, M. A.; Luth, D. and Moore, G. A. 1997. Factors affecting Agrobacteriummediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus. Plant Cell Reports. 16:745-753. doi

Kaneyoshi, J.; Kobayashi, S.; Nakamura, Y.; Shigemoto, N. and Doi, Y. 1994. A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.). Plant Cell Reports. 13: 541-545.

Moore, G. A.; Jacono, C. C.; Neidigh, J. L.; Lawrence, S. D. and Cline, K. 1992. Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants. Plant Cell Reports. 11:238-242.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-479. doi

Omura, M. and Hidaka, T. 1992. Shoot tip culture of Citrus. Bulletin of the Fruit Tree Research Station. 22:23-35.

Peña, L.; Cervera, M.; Juárez, J.; Navarro, A.; Pina, J. A. and Durán-Vila, N. 1995a. Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. Plant Cell Reports. 14:616-619.

Peña, L.; Cervera, M.; Juárez, J.; Navarro, A.; Pina, J. A. and Navarro, L. 1995b. High efficiency Agrobacterium-mediated transformation and regeneration of citrus. Plant Science. 104:183-191. doi

Peña, L.; Cervera, M.; Juárez, J.; Navarro, A.; Pina, J. A. and Navarro, L. 1997. Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration. Plant Cell Reports. 16:731-737. doi

Vardi, A.; Bleichman, S. and Aviv, D. 1990. Genetic transformation of Citrus protoplasts and regeneration of transgenic plants. Plant Science. 69:199-206. doi

Yao, J-L.; Wu, J-H.; Gleave, A. P. and Morris, B. A. M. 1996. Transformation of citrus embryogenic cells using particle bombardment of transgenic embryos. Plant Science. 113:175-183. doi