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Gamma-Ray Radiation and Sodium Azide (NaN3) Mutagenic Efficiency in Rice

DOI: http://dx.doi.org/10.13082/1984-7033.v01n04a02

http://www.sbmp.org.br/cbab/siscbab/ 

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Akihiko Ando1 & Ricardo Montalván2

 

Abstract: Rice (Oriza sativa L.) cultivar IAC-1246 seeds were treated with 10, 15, 20 and 30 Krads of gamma-rays and sodium azide (SA) concentrations of 0.5, 1.0 and 5.0 mM to study their efficiency in inducing chlorophyll mutations. Combined treatments incremented the damages when compared to single treatments, especially for seedling height and M1 panicles fertility. Treatments with SA showed a higher frequency of chlorophyll mutations than the gamma-ray treatments, both in single and combined treatments. The gamma-ray spectrum was different for the various types of mutations, whether in individual or combined treatments. The SA efficiency was higher than that of gamma - rays at 1.0 and 5.0 mM concentrations and lower at 0.5 mM. On average, the additive effect of the mutagenic combinations was more evident than the frequencies of the mutations.

Key words: induced mutation, breeding

 

1 Departmento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Caixa Postal 83, CEP 13400-970, Piracicaba - SP, Brasil
2 Embrapa Soja, Caixa Postal 231. CEP 86001-970, Londrina-Pr, Brasil. E-mail: ricardois@operamail.com

 

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