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Use of RAPD to Aid Selection in Common Bean Backcross Breeding Programs

DOI: http://dx.doi.org/10.13082/1984-7033.v01n04a04

http://www.sbmp.org.br/cbab/siscbab/ 

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Wilhelm E. Hagiwara1, João B. Santos1 & Sidney L. M. Carmo1

 

Abstract: Aiming at using RAPD markers to accelerate the recovery of desirable phenotypes in backcross programs, 167 common bean plants belonging to BC1, BC2 and BC3 resistant to the 2047 race of the fungus Colletotrichum lindemuthianum were selected after inoculation. The DNA of those plants and of the parents G2333 (donor), ESAL696 and CI140 (recurrents) was extracted and 70 polymorphic bands were obtained using 34 primers, through the RAPD procedure. Genetic similarities between BC plants and parents for each group were estimated. It was verified that RAPD was efficient in estimating genetic diversity among genotypes, as well as parentage for the BC1F4 and BC2F1 populations in relation to their parents, according to their genealogy. The efficiency of the genetic similarity for determining the parentage of the backcross populations in relation to their parents confirms its usefulness in selecting plants more similar to the recurrent parent, even in BC1.

Key words: Phaseolus vulgaris L., genetic similarity, Colletotrichum lindemuthianum

 

1 Departamento de Biologia, Universidade Federal de Lavras (UFLA), Caixa Postal 37, CEP 37200-000, Lavras, MG, Brazil. E-mail: jbsantos@ufla.br

 

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