Купить СНПЧ А7 Архангельск, оперативня доставка


Genetic Variability in Cultivated Cacao Populations in Bahia, Brazil, Detected by Isozymes and RAPD Markers

DOI: http://dx.doi.org/10.13082/1984-7033.v01n04a07



Milton M. Yamada1, Fábio G. Faleiro1, Uilson V. Lopes1, Rita C. Bahia1, José L. Pires1, Luana M. C. Gomes1 & Gilson R. P. Melo1


Abstract: The objective of this work was to study genetic variability in accessions from the CEPLAC cacao germplasm collection, which constituted the parents of hybrids recommended by CEPLAC, to infer about the diversity of cacao plantations in Bahia. The 21 clones or accessions utilized were SIC, SIAL (local selections) groups and ICS, IMC, PA, SCA, TSA and UF groups (foreign selections). Molecular markers used were isozymes (MDH, IDH, and DIA enzyme systems) and RAPD (105 RAPD loci of 11 primers). Genetic similarities among accessions were determined by the NTSYS statistical package. A similarity matrix based on Dice´ s coefficient was calculated, and a dendrogram using unweighed pair-group method with arithmetic average (UPGMA) clustering was constructed. The accessions were classified into 7 groups. Four accessions (SIC 19, IMC 67, ICS 6 and ICS 8) did not cluster in any group. Other accessions formed small groups such as SCA 6, SCA 12, PA 30, PA 150 and ICS 1. The larger group was formed by 12 accessions, including SIC, SIAL, UF and TSA 644. Results indicate that the genetic base in Bahia plantations was quite narrow before using the recommended hybrids by CEPLAC. However, with the planting of hybrids, both the genetic variability and the chances of success from selection increased.

Key words: breeding, biotechnology


1 CEPLAC, Centro de Pesquisas do Cacau (CEPEC), Seção de Genética, Laboratório de Biotecnologia, Caixa Postal 7, 45600-000, Itabuna, Bahia, Brasil. E-mail: macoto@cepec.gov.br


Literatura Citada

Anonymous. 1989. Melhoramento genético avança na pesquisa agrícola. Difusão Agropecuária. 1(1): 24-26.

Araújo, I.S.; Bahia, R.C.; Santos, R.F.; Faleiro, F.G. and Ahnert, D. 2000. Otimização da extração e amplificação de DNA de Theobroma cacao L. visando a obtenção de marcadores moleculares RAPD. Genetics and Molecular Biology. 23 (Suppl.): 219-220.

Bartley, B.G.D.; Monteiro, W.R. and Carletto, G.A. 1982.Comportamento dos clones introduzidos como progenitores de híbridos na Bahia. p.703-712. In: Atas da 8a Conferência Internacional de Pesquisas em Cacau, Cartagena, Colombia, 1981. Cartagena, Colombia.

Cascardo, J.C.; Pires, J.L. and Figueira, A. 1993. Estimating genetic diversity of cacao in Southern Bahia using RAPDs. p.47. In. Annals of the Brazilian Plant Biotechnology Meeting. REDBIO, Brasília.

Cheesman, E.E. 1944. Notes on the nomenclature, classification and possible relationship of cacao populations. Tropical Agriculture. 21: 144-159.

Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus. 12: 13-15.

Mariano, A.H. and Bartley, B.G.D. 1979. Compor-tamento das seleções baianas na produção de híbridos de cacaueiros. p.527-539. In: Atas da 7a Conferencia Internacional de Pesquisas em Cacau, Douala, Camarões, 1979. Douala, Camarões.

Marita, J.M.1998. Characterization of Theobroma cacao using RAPD marker based estimates of genetic distance and development of a core collection to maximize genetic diversity. M.S. Thesis. University of Wisconsin, Madison.

Monteiro, W.R.; Pires, J.L. and Pinto, L.R.M. 1995.Variedade Theobahia. Informação e Difusão. Nova Série. 1:1-2.

Monteiro, W.R.; Carletto, G. A. and Bartley, B.G.D.1988. Avaliação da capacidade combinatória de clones de cacaueiro. p.227-232. In: Atas da 9a Conferência Internacional de Pesquisas em Cacau. 1985, Lomé, Togo.

Sambrook, J.; Fritsch, E.F. and Maniats, T. 1989. Molecular cloning: a laboratory manual. 2.ed. Cold Spring Harbor, New York. 653p.

Toxopeus, H. 1987. Planting material. p.81-92. In: Wood, G. A R. and Lass, R. A Cocoa. Longman, London.

Rolf, F.J. 1993. NTSYS-pc. Numerical taxonomy and multivariate analysis systems, version 1.80, Exeter Software, New York.

Vello, F.; Mariano, A. H. ; Garcia, J.R.; Nascimento, T.F. and Magalhães, W.S. 1969. O programa de melhoramento genético do cacau na Bahia. p.43-55. In: Memórias da 2a Conferência Internacional de Pesquisas em Cacau. 1967. Bahia, Brasil.

Vello, F.; Garcia,J.R. and Magalhães, W. S. 1972. Production and selection of cacao hybrids in Bahia. p.38-56. In: Proceedings of the 4th International Cacao Research Conference, St. Augustine, Trinidad, 1972.

Yamada, M.M. and Guries, R.P. 1989. A manual for starch gel electrophoresis: new chocolate lovers edition. Staff paper # 39. College of Agricultural and Life Sciences. University of Wisconsin, Madison. 22p.

Yamada, M.M. and Guries, R.P. 1994.Variação genética de três sistemas isoenzimáticos em clones de cacau (Theobroma cacao) da série Parinari. Agrotrópica. 6: 27-29.

Yamada, M.M.; Bartley, B.G.D., Lopes, U. V. and Pinto, L.R.M. 1996. Herança do fator compatibilidade em Theobroma cacao. II. Relações fenotípicas em genótipos adicionais do grupo Parinari (PA). Agrotrópica. 8: 51-