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Viabilidade Celular de Cultura de Linfócitos Tratados com Annona muricata L.

DOI: http://dx.doi.org/10.15602/1983-9480/cmbs.v12n24p95-101

https://www.metodista.br/revistas/revistas-ipa/index.php/EUM/index 

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Caroline Dani1,2,3, Joanna C. Aginoni1, Caroline Calloni1, Mirian Salvador1 & Patricia D. S. Spada1,3

 

Resumo: O interesse em pesquisas com agentes antioxidantes naturais, tanto para fins terapêuticos, como na área de tecnologia de alimentos vem aumentando, uma vez que, o uso de antioxidantes sintéticos pode apresentar efeitos adversos, ou ainda não bem estabelecidos. Há evidências consistentes da ação protetora dos antioxidantes naturais contra os efeitos deletérios do estresse oxidativo decorrente do ataque dos radicais livres aos sistemas celulares. Muitos antioxidantes utilizados atualmente estão presentes na dieta humana, e possuem em sua composição compostos fenólicos, entre eles os flavonóides. Sendo assim, os flavonóides, passaram a ter seu mecanismo de ação investigado, procurando relacioná-lo com as propriedades benéficas apresentadas por esses compostos. A Annona muricata L. (gravioleira), é uma espécie cultivada em regiões tropicais das Américas, apresenta uma nova classe de fitoquímicos, as acetogeninas anonáceas, que entre outras atividades tem demonstrado ser antitumoral, pesticida, inseticida, antibacteriana, antiparasitário e efeito imunossupressor. Em vista disso, o objetivo desse estudo objetivo foi avaliar a influência das folhas (infusão e liofilizadas) e polpa de graviola congelada na viabilidade celular de cultura de linfócitos tratados com peróxido de hidrogênio, assim como a atividade antioxidante frente ao radical DPPH. O tratamento prévio com as amostras de gravioleira mostrou significativo aumento na viabilidade dos linfócitos comparando-se com o controle do agente estressor (H2O2). Foi verificada uma correlação positiva entre a atividade antioxidante e o conteúdo de polifenóis totais (r= 0,896; p≤0,05). Os dados obtidos com este estudo prévio indicam que os derivados da gravioleira apresentam importante atividade antioxidante frente ao radical DPPH, e também aumento na viabilidade celular, indicando minimização nos danos oxidativos.

Palavras-chave: Annona muricata L. – Antioxidante – Peróxido de hidrogênio – Viabilidade celular

 

Abstract: Research with natural antioxidants, for therapeutic purposes and application for food technology area, is increasing, since the use of synthetic antioxidants may have adverse effects, or not well established yet. There is consistent evidence of the protective action of natural antioxidants against the damaging effects of oxidative stress resulting from the damage of free radicals to cellular systems. Many antioxidants currently used are present in the human diet, and have in their composition, phenolic compounds such as flavonoids. Thus, flavonoids, have been given its mechanism of action investigated, trying to relate it with the beneficial properties provided by these compounds. The Annona muricata L. (gravioleira) is a species cultivated in the tropics of the Americas, presents a new class of phytochemicals, the annona-ceae acetogenins, which among other activities has proved to be malignant disease, pesticide, insecticide, antibacterial, antiparasitic and immunosuppressive effect. The objectives of this study were evaluate the influence of leaves (infusion and lyophilized) and frozen soursop pulp in viability of lymphocyte culture cells treated with hydrogen peroxide, and the antioxidant activity to scavenger DPPH radical. Pretreatment with samples gravioleira showed significant increase in the viability of lymphocytes compared with the control of the stressor agent (H2O2). It was observed a positive cor-relation between antioxidant activity and total polyphenol content (r = 0.896, p ≤ 0.05). The data obtained from this preliminary study indicate that derivatives of gravioleira present a significant antioxidant activity against the DPPH radical and also increased cell viability, indicating in minimizing oxidative damage.

Key words: Annona muricata -Antioxidant - Hydrogen peroxide – Cell viability

 

1 Laboratório de Estresse Oxidativo e Antioxidantes, Universidade de Caxias do Sul, RS
2 Centro Universitário Metodista, IPA
3 Curso de Biomedicina, Faculdade da Serra Gaúcha, RS. *Instituto de Biotecnologia, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas, 1130 – Bairro Petrópolis, Caxias do Sul, RS – 95070-560. E-mail: pspada@ucs.br

 

Literatura Citada

ABDILLE, M.H. et al. Antioxidant activity of the extracts from Dillenia indica fruits. Food Chemistry, v. 90, p. 891-896, 2005. doi

CASSILETH, B. Complementary therapies, herbs, and other OTC agents. Oncology, v. 22, n. 10, p.1202-1208. 2008.

COLMAN-SAIZARBITORIA, T.; MONTILLA, L.; RODRIGUEZ, M.; CASTILLO, A. HASEGAWA, M. Xymarginatin: a new acetogenin inhibitor of mitochondrial electron transport from Xylopia emarginata Mart., Annonaceae. Revista Brasileira de Farmacognosia, v. 19, n. 4, p. 871-875, 2009. doi

HEIM, K.E.; TAGLIAFERRO, A.R.; BOBILYA, D.J. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. The Journal of Nutritional Biochemistry , v. 13, p. 572-584, 2002. doi

HERTOG, M.G.L. et al. Antioxidant flavonols and ischaemic heart disease in a Welsh population of men. The Caerphilly study. American Journal of Clinical Nutrition, v. 65, p. 1489-1494, 1997.

ISHIGE, K. et al. Flavonoids protect neuronal cells from oxidative stress by three distinct mechanisms. Free Radical Biology and Medicine, v. 30, p. 433-446, 2001. doi

KATSUBE, N. et al. Induction of apoptosis in cancer cells by bilberry (Vaccinium mirtillus) and the an-thocyanins. Journal of Agricultural and Food Chemistry , v. 51, p. 68-75, 2003. doi

LIU, H.X.; HUANG, G.R.; ZHANG, H.M.; WU, J.R.; YAO, Z.J. Annonaceous acetogenin mimics bearing a terminal lactam and their cytotoxicity against cancer cells. Bioorganic & Medicinal Chemistry Letters, v. 17, n. 12, p. 3426-30, 2007. doi

MOSMANN, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, v. 65, p. 55-63, 1983. doi

NAJAFZADEH, M.; REYNOLDS, P.D.; BAUMGARTNER, A.; ANDERSON, D. Flavonoids inhibit the genotoxicity of hydrogen peroxide (H(2)O(2)) and of the food mutagen 2-amino-3-methylimadazo[4,5-f]-quinoline (IQ) in lymphocytes from patients with inflammatory bowel disease (IBD). Mutagenesis, v. 24, p. 405-11, 2009. doi

NESS, A.R.; POWLES, J.W. Fruit and vegetables, and cardiovascular disease: a review. International Journal of Epidemiology, v. 26, n. 1, p. 1-13, 1997. doi

SACRAMENTO, C. K.; FARIA , J. C., CRUZ, F. L.; BARRETTO, W. S.; GASPAR, J. W.; LEITE, J. B. V. Caracterização física e química de frutos de três tipos de gravioleira (Annona Muricata L.). Revista Brasileira de Fruticultura, v. 25, n. 2, p. 329-331, 2003. doi

SHAHIDI, F. Natural Antioxidants: An Overview “in” Natural Antioxidants Chemistry, Health Effects, and Applications. AOCSPress: Champaign, Illinois, p. 1-11. 1996.

SINGLETON, V.L.; ROSSIJ.A. Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. American Journal of Enology and Viticulture, v. 16, p. 144–158, 1965.

SPADA, P. D.S.; SOUZA, G. G. N.; BORTOLINI, G. V., HENRIQUES, J. A.P.; SALVADO, M. Antioxidant, Mutagenic, and Antimutagenic Activity of Frozen Fruits. Journal of Medicinal Food, v. 11, n. 1, p. 144–151, 2008. doi

STOCLET, J.C. et al. Vascular protection by dietarypolyphenols. Journal of Pharmaceutical Sciences, v. 500, p. 299-313, 2004.

WANG, J.; MAZZA, G. Effects of anthocyanins and other phenolic compounds on the production of tumor necrosis factor alpha in LPS/IFN-gamma-activated RAW 264.7 macrophages. Journal of Agricultural and Food Chemistry , v. 50, p. 4183-4189, 2002. doi

YAMAGUCHI, T.; TAKAMURA, H.; MATOBA, T.C.; TERAO, J. Free radical scavenging activity of grape seed extract and antioxidants by electron spin resonance spectrometry in an H2O2/NaOH/DMSO system. Bioscience, Biotechnology and Agrochemistry, v. 62, p. 1201–1204, 1998.

YUAN, S.S.; CHANG, H.L.; YEH, Y.T.; KAO, Y.H.; UN, K.H.; WU, Y.C.; SU, J.H. Annonacin, a mono-tetrahydrofuran acetogenin, arrests cancer cells at the G 1 phase and causes cytotoxicity in a Bax- and caspase-3-related pathway. Life Sciences, v. 72, p. 2853-2861, 2003. doi