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Correlação entre a Avaliação Microscópica e a Leitura por Laserfluorescência de Lesões de Manchas Brancas em Dentes Bovinos: Estudo in vitro

DOI: http://dx.doi.org/10.15603/2176-1000/odonto.v18n36p31-39

https://www.metodista.br/revistas/revistas-ims/index.php/O1/index 

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Paula D. Matheus1, Carina F. Demito2, Paula C. Scheibel3, S. Jay Bowman4 & Adilson L. Ramos5

 

Resumo: Objetivo: avaliar a correlação entre as leituras de descalcificação artificial de esmalte in vitro por meio do DIAGNOdent® e pela microscopia óptica com luz polarizada. Metodologia: foram utilizados 25 dentes bovinos que foram expostos a um meio artificial de cáries por uma hora, duas vezes ao dia, por 35 dias. Em seguida, procedeu-se as leituras das fluorescências utilizando o aparelho laser DIAGNOdent®. Foram registradas as leituras das áreas sem descalcificação (para calibração) e as áreas desmineralizadas artificialmente. Após as leituras da fluorescência, as regiões de leitura por laser foram cortadas em secções de aproximadamente 400μm e avaliadas por meio da microscopia de luz polarizada. Resultados: demonstraram que, embora com algum grau de variação, o sistema DIAGNOdent® foi capaz de detectar as descalcificações semelhantemente àqueles reconhecidos por microscopia de luz polarizada, estabelecendo um padrão razoável de equivalência de leituras. Conclusão: os valores médios encontrados para o DIAGNOdent® demonstraram um coeficiente de correlação de Pearson de 0,63 com relação aos valores das leituras microscópicas.

Palavras-chave: Desmineralização; Laser; Microscopia de Luz Polarizada.

 

Abstract: Aim: evaluate the correlation between the artificial in vitro enamel decalcification through DIAGNOdent® laser and through optic microscopic with polarized light.Methodology: for this research, 25 bovine teeth had been exposed to an artificial decay environment during one hour, two times a day, for 35 days. Afterwards, DIAGNOdent® laser has been used to read the fluorescence from the enemel areas without decalcification (for calibration) and to evaluate the non-mineralized artificially. After the fluorescence readings regions of laser read were cut into sections of about 400μm and evaluated by polarized light microscopy. Results: the results had demonstrated that, even with some degree of variation, the DIAGNOdent® system was capable of recognizing the decalcification, establishing regular equivalence of standard readings such as light polarized microscopy. Conclusion: the average values found for DIAGNOdent® laser had demonstrated a 0,63 coefficient of correlation of Pearson to the values of microscopically readings.

Key words: Decalcification; Laser; Polarized Light Microscopy.

 

1 Mestranda em Patologia Bucal – Faculdade de Odontologia / UFRGS
2 Especialista em Ortodontia – Universidade Estadual de Maringá
3 Mestre em Odontologia Integrada / UEM e Especialista Ortodontia / CESUMAR
4 Clinica Privada Portage, Michigan, EUA, Mestre e Especialista em ortodontia/Saint Louis University
5 Professor adjunto do Departamento de Odontologia da UEM/ Mestre (USPBauru) e Doutor (UNESP-Araraquara) em Ortodontia

 

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